Williams Gallas, Ledley King (both groin), Michael Dawson (knee and ankle) and Younes Kaboul (hamstring) are all sidelined with injury.
Right-back Vedram Corluka could play alongside Bassong, as he did in the 1-0 defeat by West Ham on Saturday.
Striker Jermain Defoe (ankle) also misses out.
Despite the injury crisis, Spurs manager Harry Redknapp said his side would be looking to attack in the Group A encounter in order to seal their first Champions League win.
"We have to go for it," stated Redknapp. "Getting a win is all important, if we get a win it sets us up nearly nicely. The group is wide open on one point each at the moment.
"Inter Milan are obviously the favourites but after that it is wide open. We have looked at a couple of Twente's games on video and they have one or two really good players.
"But at home, the crowd are up and it will be an intimidating atmosphere like against Young Boys [the Swiss side Spurs beat to reach the group stage proper] and we attacked them and got after them from the start.
"That is what we have to do in these [home] European games, especially in this group.
"In Milan you might set up differently. If you play some teams you would not be playing 4-4-2 or you would get ripped to pieces."
Meanwhile, Dutchman Rafael van der Vaart said he hoped his experience in Europe would rub off on the rest of his team.
"I have a lot of experience in the Champions League," stated 27-year-old midfielder Van der Vaart, who joined Spurs from Real Madrid in the summer.
"I have played with Ajax in the Champions League, and Hamburg and Madrid. I hope I can help the team and I'm sure I can."
"He will do well for us," said Redknapp. "I have been really impressed with him. He is a good lad, he wants to play, he wants to win and has got a good attitude.
"He's got 80 caps for his country and you don't get them if you are a mug."
Van der Vaart's importance to the side has increased as winger Aaron Lennon has suffered a slump in form that has seen him lose his place in the England squad.
"I think it's about confidence," Redknapp explained. "You've got to keep working with him because, when he's on his game he's a top-class wide player, so we need to get him back to doing that again."
Tottenham and Twente both drew their opening group games 2-2 - Spurs gave away a two-goal lead away to Werder Bremen while Twente managed a creditable point at home to reigning champions Inter Milan.
» Sky Sports News Links
» Hello....the site is still alive!
» 17 million deal for Musacchio
» How to Block Adverts on this Site !
» This is Our Season
» 'appy New Season
» Time to say goodbye
» Crouchinio